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##### Asked by: Kang Natarie

business and finance pharmaceutical industry# How do you calculate dilutions?

Last Updated: 19th January, 2020

**dilute**solution from a stock solution, you can use the formula: C

_{1}V

_{1}= C

_{2}V

_{2}where: V

_{1}= Volume of stock solution needed to make the new solution. C

_{1}= Concentration of stock solution.

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In this regard, how do you do dilutions?

To **make** a **dilution**, you simply add a small quantity of a concentrated stock solution to an amount of pure solvent. The resulting solution contains the amount of solute originally taken from the stock solution but disperses that solute throughout a greater volume.

Also Know, what is the difference between dilution and dilution factor? 1.**Dilution** is a process with no formula while a diluents **factor** requires a formula to get the answer. 2.**Dilution** is the process of reducing the amount of concentration while the diluents **factor** is the ratio of the final volume over aliquot volume.

Hereof, how do you calculate antibody dilution?

So take 3 uL from your Primary **antibodies** stock vial and add into 3000 uL (3 mL) of PBS or any other diluent as per your choice. So this is yours 1:1000 **dilution** in total of 3 ml. To confirm this **calculation**, just divide 3000 / 3 which gives 1000 which is our desired **dilution** factor here.

What do you mean by dilution?

**Definition** of **Dilution**. **Dilution** is the process of reducing the concentration of a solute in solution, usually simply by mixing with more solvent. Example 1: **You** can add water to concentrated orange juice to **dilute** it until it reaches a concentration that is pleasant to drink.