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Asked by: Kang Natarie
business and finance pharmaceutical industryHow do you calculate dilutions?
In this regard, how do you do dilutions?
To make a dilution, you simply add a small quantity of a concentrated stock solution to an amount of pure solvent. The resulting solution contains the amount of solute originally taken from the stock solution but disperses that solute throughout a greater volume.
Hereof, how do you calculate antibody dilution?
So take 3 uL from your Primary antibodies stock vial and add into 3000 uL (3 mL) of PBS or any other diluent as per your choice. So this is yours 1:1000 dilution in total of 3 ml. To confirm this calculation, just divide 3000 / 3 which gives 1000 which is our desired dilution factor here.
Definition of Dilution. Dilution is the process of reducing the concentration of a solute in solution, usually simply by mixing with more solvent. Example 1: You can add water to concentrated orange juice to dilute it until it reaches a concentration that is pleasant to drink.