Asked by: Abderazak Kaltwasser
medical health diabetes

How do you make a 20% glucose solution?

Last Updated: 5th June, 2021

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Procedure
  1. Measure out 1.5L of DI water into 2L flask on hot plate.
  2. Add stir bar and begin spinning.
  3. Measure out 400g of glucose powder.
  4. Stir until dissolved.
  5. Bring total volume up to 2L using 2nd graduate cylinder.
  6. Add back to flask for mixing.
  7. Dispense 100mL of glucose solution into 20 100mL glass bottles.

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In this regard, how do you make a 1% glucose solution?

To work out how much glucose you need to make a solution of a given percent, multiply (mass/volume) by volume, bearing in mind that 1 g in 100 ml is a 1 percent solution. For this example, if you want to make a total solution of 500 ml of 20 percent glucose, multiply (20/100) by 500.

Also, what is a 20% solution? 20% by mass means 20 g of the NaOH is dissolved in 100 g of solution. What is the meaning of 20% mass by volume solution? 20% mass by volume means 20 g of the NaOH is dissolved in 100 mL of the solution.

In this way, how do you make a glucose solution?

Procedure

  1. Weight out 20g of glucose (dextrose)
  2. Add to 70ml of H2O.
  3. Dissolve by stirring. May use some heat.
  4. Once the sugar has dissolved, bring the volume of the mix up to 100ml total.
  5. Autoclave.

How do you make a 10% sucrose solution?

Dissolve 10 grams sugar in 80 ml water. After the sugar is completely dissolved, adjust the volume to 100 ml. 10% sugar and 90% water by mass.

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What is a 1% solution?

A one percent solution is defined as 1 gram of solute per 100 milliliters final volume. For example, 1 gram of sodium chloride, brought to a final volume of 100 ml with distilled water, is a 1% NaCl solution. To help recall the definition of a 1% solution, remember that one gram is the mass of one milliliter of water.

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How do you make a 30% sucrose solution?

30% sucrose solution: To make 100 ml, dissolve 30 g sucrose in about 70 ml 0.1M PBS solution; once dissolved, make up volume to 100 ml total by adding 0.1M PBS solution. Store at 4oC, do not use once contaminated with microorganisms (this can be prevented by filter sterilisation).

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What is glucose control solution?

Control solution is a glucose solution that manufacturers produce with a set amount of glucose. Each has a different concentration of glucose, allowing you to check for test accuracy at low, normal and high glucose levels for any given batch of test strips.

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How do you make a 5% dextrose solution?

– To make a 2.5% solution add 50mL of 50% dextrose (or 25g dextrose) to a 1L bag of fluids. – To make a 5.0% solution add 100mL of 50% dextrose (or 50g dextrose) to a 1L bag of fluids.

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What glucose is 40%?

Glucose 40% w/v has an osmolarity of 2220 mOsmol/L. Glucose 40% w/v is for use in admixtures to provide temporary relief from the symptoms of increased intracranial pressure and hypoglycaemic coma and is also indicated for the supplementation of energy in parenteral nutrition.

Esma Hrula

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How do you make a 40 sugar solution?

Suspend 40 grams in 60 ml of purified / distilled water. Heat to boiling to dissolve the sucrose completely. If required make the volume to100 ml with distilled water. Dispense in suitable container and sterilize by autoclaving at 115°C for 10 min.

Zacarias Hellwich

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How do you make a 40 solution?

To prepare a 40%NaOH solution you weigh 40 gm NaOH and dissolve it in 100 ml water. To prepare 1 M you dissolve the molecular weight of it in one L. The molecular weight of NaOH is 23+16+1=40 gm.

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What is a 1 1 solution?

1:1 is just a common way to express the composition of a solution. In this case mixing equal amount of two components. Most of the time they mean volume but sometimes it's weight. E.g. a 1:1 MeOH:water (v/v) solution is made by mixing an equal volume of water with methanol.

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How do you make a 12.5 glucose solution?

For example, to prepare 100ml of 10% dextrose from 5% dextrose and 25% dextrose, add 5x10-25=25ml of 25% dextrose to the remaining volume, i.e. 100- 25 =75 ml of 5% dextrose. To prepare 100 ml of 12.5% dextrose, add 5x12. 5-25=37.5ml of 25% dextrose to 62.5 ml (100-37.5) of 5% dextrose. 2.

Deepak Atxurra

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How do you find the concentration of glucose?

Determine the concentration of glucose in each Kool-Aid sample either by calculating it or using the graph. To calculate it, divide its absorbance by the slope of the standard curve. If you did not have to redraw the standard curve graph, you can find the slope of the standard curve in the equation of a line.

Georgiann Ebdon

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How do you make a standard glucose curve?

  1. a) Standard stock solution of glucose. Standard stock solution was prepared by dissolving 100 mg of glucose in 100 mL of distilled water and working standard was prepared by diluting 10 mL of stock solution to 100 mL with distilled water.
  2. b) Preparation of glucose oxidase peroxidase reagent.
  3. Preparation of standard curve.

Florica Anastasio

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Chuck Palomas

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What is diluent volume?

A dilution solution contains solute (or stock solution) and a solvent (called diluent). Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1 ÷ 20 = 1.5 mL of stock solution.

Xiaoling Gartler

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What is a 1/10 dilution?

For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one "part" of the 1M solution with nine "parts" of solvent (probably water), for a total of ten "parts." Therefore, 1:10 dilution means 1 part + 9 parts of water (or other diluent).

Ghania Pallavan

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How do you make a 5% solution of NaCl?

A solution that contains five percent salt, or NaCl, contains five ounces of NaCl per 100 ounces of total solution, where “total solution” refers to the combined weight of the NaCl and water together. Weight out about 199 grams, or seven ounces, of table salt and transfer the salt into an empty gallon container.

Bismark Austin

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How do you make 5% HCl?

To put long story short, add 640 ml of water to 100 ml of a solution of 37% HCl in water and mix them well, to achieve a final solution with volume of 740 ml whereby the HCl is 5% only.

Odei Ehrlich

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Is 20 sucrose solution hypertonic?

The sucrose solution is hypertonic to the water – it is a more concentrated solution. A less concentrated solution is hypotonic to a more concentrated solution, while two solutions at the same concentration are isotonic.

Shuying Aladid

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How do you get 10% HCl from 37 %?

Re: Preparing 10% HCl
In 37% conc HCl,mass of HCl in 1000ml=1.19g/ml x1000ml x37%=440.3g. mass of HCl in 100ml=44.03g. 10/44.03 x 100ml = 22.7ml of conc HCl. Add water to make up to 100ml to get 10%w/v HCl.