Asked by: Marilda Paholkov
medical health infectious diseases

Is SPS an anticoagulant?

Last Updated: 18th April, 2020

15
Sodium polyanethole sulfonate (SPS) is the most common anticoagulant used in commercial blood culture bottles. SPS has been shown to function as an anticoagulant (4) and as an inhibitor of humoral and cellular elements that might interfere with bacterial growth.

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Considering this, what is SPS additive?

Additive: anticoagulant SPS (Sodium Polyanetholsulfonate) & ACD (acid citrate dextrose) What additive does: Prevents the blood from clotting and stabilizes bacterial growth.

Similarly, what is the liquid in blood culture bottles? A minimum of 10 ml of blood is taken through venipuncture and injected into two or more "blood bottles" with specific media for aerobic and anaerobic organisms. A common medium used for anaerobes is thioglycollate broth.

Also, what is the purpose of sodium Polyanethole sulfonate?

Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors.

What is the solution in blood culture bottles?

Columbia Broth is particularly recommended for blood culture because of its ability to grow a wide range of microorganisms. Columbia Broth with Increased Cystein, Hemin and Menadione provide improved recovery of anaerobic microorganisms from blood specimens.

Related Question Answers

Hyacinth Ruiz Henestrosa

Professional

What additives are in each tube?

Tube cap color Additive
Light-blue 3.2% Sodium citrate
Red or gold (mottled or "tiger" top used with some tubes is not shown) Serum tube with or without clot activator or gel
Green Sodium or lithium heparin with or without gel
Lavender or pink Potassium EDTA

Ismahane Bachhofen

Professional

What does SPS stand for in phlebotomy?

sodium polyanethol sulfonate

Banta Schonau

Professional

What blood test go in which tubes?

Use: EDTA whole blood or plasma. Send whole blood in a royal blue-top tube. Send plasma in a plastic transport tube labeled “Plasma, EDTA from royal blue.” Tan-top tube: Contains sodium EDTA for blood lead analysis.

Christina Gagg

Explainer

What additive is in a SST tube?

Collection Tubes for Blood Specimens
Gold or Red-Gray Marble Stopper (two-types) - SST, Serum Separator Tube
Additive: Polymer gel and powdered glass clot activator
Specimen Type: Serum
Draw Amount: 5.0 mL/8.5 mL
Inversions: 5

Devid Ruiz Valdepeñas

Explainer

What additives are in royal blue tubes?

Royal Blue acid washed tubes are available in No Additive clot tubes for serum collection as well as EDTA and Sodium Heparin additives for plasma collections.

Anja Ceron

Explainer

What is the order of blood collection tubes?

The draw order for specimen tubes is as follows:
Gold SST (Plain tube w/gel and clot activator additive) Green and Dark Green (Heparin, with and without gel) Lavender (EDTA) Pink - Blood Bank (EDTA)

Lily Nisa

Pundit

What is a SST blood test for?

The short Synacthen test is a test of adrenal insufficiency which can be used as a screening procedure in the non-critically ill patient. The test is based on the measurement of serum cortisol before and after an injection of synthetic ACTH (also known as tetracosactrin).

Nermine Poenisch

Pundit

What happens if the proper order of draw is not followed?

If the order of draw is not followed, then the additives in one tube can be transferred to another, causing inaccurate results and cross-contamination. Inaccurate results may lead to misdiagnosis which may prevent patients from receiving the correct treatment.

Adoralina Feldkircher

Pundit

How do you know if a blood culture is contaminated?

Clues that may help to differentiate contamination from bacteremia include identity of the organism, number of positive culture sets, number of positive bottles within a set, time to growth, quantity of growth, clinical and laboratory data, source of culture, and automated classification using information technology.

Valentyn Lindenkreuz

Pundit

Which blood culture bottle do you fill first?

If collecting from an adult or adolescent, the aerobic bottle (blue top) must always be inoculated first to avoid the release of air from the syringe into the anaerobic bottle. Dispose of the needle and syringe appropriately (e.g. in a sharps bin).

Bezza Reimpell

Pundit

How long does it take for a blood culture?

2 to 3 days

Tiffanie Schein

Teacher

How much blood is in a blood culture bottle?

Ten ml of blood is optimal in each blood culture bottle. Do not overfill the bottles as this can lead to false-positive results due to excessive WBC's. If less than 10 ml is obtained, 5 ml is placed into the aerobic (blue) blood culture bottle and the rest is placed into the anaerobic (purple) bottle.

Nacor Yuhimuk

Teacher

What does it mean when a blood culture is positive?

A blood culture is a test that checks for foreign invaders like bacteria, yeast, and other microorganisms in your blood. Having these pathogens in your bloodstream can be a sign of a blood infection, a condition known as bacteremia. A positive blood culture means that you have bacteria in your blood.

Manuele Eagle

Teacher

How much do blood culture bottles cost?

The cost associated with negative blood cultures and those with contaminants was $34,950 ($50 per culture for 699 cultures); the cost for 11 positive cultures was $1100 ($50 per culture plus $50 for antimicrobial susceptibility testing for 11 cultures).

Luftolde Kilmurry

Teacher

What does a contaminated blood culture mean to the patient?

Blood culture contamination is defined as the recovery of normal skin flora (coagulase-negative staphylococci, Propionibacterium spp., Aerococcus, Micrococcus, Bacillus spp. [not B. anthracis], Corynebacterium spp. [diphtheroids], and alpha-hemolytic streptococci) from a single blood culture.

Harinder Bouakka

Reviewer

What is BD bactec used for?

The BD BACTEC bottle portfolio provides a full line of blood culture media developed specifically for the detection of aerobes, anaerobes, yeast, fungi and mycobacteria to help improve time to detect and organism recovery2 from both adult and pediatric patients.

Som Erquicia

Reviewer

How long does it take to get the results of a culture?

A urine culture is a test to find germs (such as bacteria) in the urine that can cause an infection. Urine culture results are usually ready in 1 to 3 days. But some germs take longer to grow in the culture. So results may not be available for several days.

Anjara Carril

Reviewer

How do you get bacteremia?

Bacteremia. Bacteremia is the presence of bacteria in the bloodstream. Bacteremia may result from ordinary activities (such as vigorous toothbrushing), dental or medical procedures, or from infections (such as pneumonia or a urinary tract infection).

Claudio Guapo

Reviewer

How many blood cultures are needed?

The results of this study indicate that two blood cultures in a 24-h period will detect approximately 90% of bloodstream infections in adults. To achieve a detection rate of >99%, as many as four blood cultures may be needed.

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18th April, 2020

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