Asked by: Tomislav Heinkelmann
medical health medical tests

What are SPS tubes?

Last Updated: 2nd June, 2020

Where are SPS (sodium polyanethol sulfonate) tubes used? A. SPS tubes are used for blood culture specimen collection in microbiology. Eight gentle tube inversions will prevent the blood from clotting. The blood can remain in the SPS tube for two to four hours before it has to be transferred to a blood culture bottle.

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People also ask, what is SPS in phlebotomy?

Sodium polyanethole sulfonate (SPS) is the most common anticoagulant used in commercial blood culture bottles. SPS has been shown to function as an anticoagulant (4) and as an inhibitor of humoral and cellular elements that might interfere with bacterial growth.

Additionally, what are the different blood collection tubes? Red-Top Tube - This tube has no anticoagulant and is used for many chemistry tests, drug levels, and blood bank procedures. Navy Blue-Top Tube - There are two general types - one with K2 EDTA and one with no anti-coagulant. Serum Separator Tube (SST®) - This tube contains a clot activator and serum gel separator.

Subsequently, one may also ask, what is SPS additive?

Additive: anticoagulant SPS (Sodium Polyanetholsulfonate) & ACD (acid citrate dextrose) What additive does: Prevents the blood from clotting and stabilizes bacterial growth.

What is gray top tube used for?

Grey-top tube (potassium oxalate/sodium fluoride) This tube contains potassium oxalate as an anticoagulant and sodium fluoride as a preservative – used to preserve glucose in whole blood and for some special chemistry tests.

Related Question Answers

Kattie Joy


What tests go in what color tubes?

Green top tube with sodium or lithium heparin: used for plasma or whole blood determinations. EDTA tubes: includes Lavender top, Pink top (used for blood bank testing), Tan top (used for lead testing), and Royal Blue top with EDTA (used for trace metal whole blood or plasma determinations).

Rambha Schwabeland


What do the colors of blood test tubes mean?

The tests each bottle is used for are the same: the purple one is for cell count, the yellow one is for electrolytes, albumin and LDH, the grey one is for glucose, and blood culture bottles can be used for fluid cultures.

Yosu Venkatesann


What color is BMP tube?

Green Tube/Plasma: Centrifuge after collection. Gold Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Amia Saraf


What blood tube colors are for which test?

Tube cap color Additive Common laboratory tests
Green Sodium or lithium heparin with or without gel Stat and routine chemistry
Lavender or pink Potassium EDTA Hematology and blood bank
Gray Sodium fluoride, and sodium or potassium oxalate Glucose (especially when testing will be delayed), blood alcohol, lactic acid

Sigita Jawurek


What color tube is used for TSH?

Specimen type: Plasma or serum
Preferred collection container: Stat/Line draws: 3 mL green/yellow-top (plasma separator) tube Routine requests/off-site specimens: 3.5 mL gold-top (serum separator) tube

Avelinda Mendiola


Why is SPS used in blood cultures?

Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture media. The addition of SPS to experimental blood cultures inoculated with small numbers of a variety of organisms caused a striking increase in recovery of these organisms.

Rafela Irarzabal


Yaimara Piasecki


Can you pour blood from one tube to another?

Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings (see illustrations at end).

Earnest Herrasti


What is the order of draw?

Order of draw is the tube sequence a certified phlebotomist needs to follow while collecting blood. Each tube is differentiated by the tube additive and color. A trained phlebotomist using the correct order of draw ensures they obtain a quality sample to be used for diagnostic purposes to provide accurate results.

Dorys Vikhlyaev


What additives are in royal blue tubes?

Royal Blue acid washed tubes are available in No Additive clot tubes for serum collection as well as EDTA and Sodium Heparin additives for plasma collections.

Tami Urbiztondo


What color tube has no additives?

Royal Blue No additive (serum); special glass and stopper material 7.0 mL
Red No additive 7.0 mL
Light Blue 3.2% Sodium Citrate 4.5 mL
Gold Top (Serum Separator, "SST") Contains separating gel and clot activator 6.0 mL

Tiberio Tzagareli


What additive is in a tiger top tube?

Tiger-top tube (serum separator): Tube does not contain an anticoagulant but does contain a clot activator and serum separator gel. This tube is used for collecting serum. Yellow-top tube (ACD): Tube contains acid citrate dextrose as an anticoagulant.

Kamal Werthmanns


What is lithium heparin?

Heparin VACUETTE® heparin tubes are coated on the inside wall with spray-dried lithium, ammonium or sodium heparin and are used to determine analytes in clinical chemistry. The additive acts as an anticoagulant, and blocks the clotting cascade.

Ghali Port


How do you remember Order of phlebotomy?

“What mnemonic do you use to remember or teach the proper order of draw? (example: "Studious Boys Rarely Get Low Grades" = Sterile, blue, red, green, lavender, gray.)”

Maite Jerico


What is a yellow top blood tube for?

Yellow-top tube (ACD): Tube contains acid citrate dextrose as an anticoagulant. This tube is used for the collection of whole blood for special studies. Royal blue-top tube: There are 2 types of tubes; one contains the anticoagulant EDTA and the other does not contain an anticoagulant.

Lledo Costel


What is EDTA vial?

Purple or lavender - contains EDTA (the potassium salt, or K2EDTA). This is a strong anticoagulant and these tubes are usually used for complete blood counts(CBC) and blood films. Lavender top tubes are generally used when whole blood is needed for analysis.

Leopoldina Baraks


How do vacutainer tubes work?

When a Vacutainer tube is inserted into the holder, its rubber cap is punctured by the inner needle and the vacuum in the tube pulls blood through the needle and into the tube. Blood collection tubes expire because over time the vacuum is lost and blood will not be drawn into the tube when the needle punctures the cap.

Jairo Ramamurthy


What is the white stuff at the bottom of a blood vial?

Fluoride is a substance that inhibits glycolysis inside the blood cells. This prevents the plasma glucose levels to drop inside the tube due to glycolysis by the cells. The resulting product is plasma that is specifically used for the measurement of glucose.

Adams Sastosain


How much blood does a vacutainer hold?

-approved continuing education credits by subscribing online. Adult tubes generally hold from 3 to 10 ml of blood. Pediatric tubes usually hold from 2 to 4 ml. Tubes for fingersticks or heelsticks generally hold one half ml or less.