Asked by: Soraia Facchinettiscience genetics
What commonly serves as a DNA cloning vector?
Last Updated: 14th May, 2020
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Correspondingly, what is a vector in DNA cloning?
In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g., plasmid, cosmid, Lambda phages). A vector containing foreign DNA is termed recombinant DNA.
Similarly, how do you clone a vector? Experimental Procedure
- Run PCR and purify the PCR product: Run PCR to amplify your insert DNA.
- Digest your DNA:
- Isolate your insert and vector by gel purification:
- Ligate your insert into your vector:
- Isolate the Finished Plasmid:
- Verify your Plasmid by Sequencing:
In respect to this, how does pBR322 work as a cloning vector?
pBR322 Plasmid pBR322 was one of the first plasmids used for the purpose of cloning. It contains genes for the resistance to tetracycline and ampicillin. Insertion of the DNA at specific restriction sites can inactivate the gene for tetracycline (an effect known as an insertional inactivation) or ampicillin resistance.
Which technique allows many copies of a specific section of DNA to be made very rapidly?
Polymerase chain reaction