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Besides, what is a DNA ladder used for?
A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel
In this way, what is a ladder in PCR?
Overview. DNA ladders consist of a set of DNA fragments of different sizes. These DNA fragments are separated and visualised as DNA bands on agarose or SDS DNA gels. DNA ladders are used during gel electrophoresis to determine both size as well as for quantification of PCR products.
The DNA molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole.