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If collecting from an adult or adolescent, the aerobic bottle (blue top) must always be inoculated first to avoid the release of air from the syringe into the anaerobic bottle. Dispose of the needle and syringe appropriately (e.g. in a sharps bin).
Thereof, what is the order of draw for blood culture?
The draw order for specimen tubes is as follows: Blood culture. Blue tube for coagulation (Sodium Citrate) Red No Gel. Gold SST (Plain tube w/gel and clot activator additive)
Moreover, when Should blood cultures be drawn?
Most guidelines state that blood specimens should be collected in the absence of antimicrobials, at or around the time of fever spikes, and a 30–60 min interval between samples has been arbitrarily recommended (Weinstein, 1996).
The aerobic bottle should be inoculated first for several reasons, including the fact that air from the tubing is pulled into the bottle compromising an anaerobic environment and if flow of blood is interrupted, most of the organisms that cause septicemia (aerobic) will be recovered.