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Blue light: The absorbance values will decrease over time as blue light is absorbed the carotenoids and chlorophyll b for photosynthesis. Thus the DCPI will become reduced and change from blue to colorless over time.
Also, how does absorbance relate to photosynthesis?
Pigments absorb light used in photosynthesis. Instead, photosynthetic organisms contain light-absorbing molecules called pigments that absorb only specific wavelengths of visible light, while reflecting others. The set of wavelengths absorbed by a pigment is its absorption spectrum.
In this manner, how is Dcpip reduced in photosynthesis?
When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction. DCPIP has a higher affinity for electrons than ferredoxin and the photosynthetic electron transport chain can reduce DCPIP as a substitute for NADP+, that is normally the final electron carrier in photosynthesis.
Ammonium hydroxide has slowed electron transfer along electron transport chain to DCPIP. Ammonium hydroxide accepts electrons and does not pass electrons along electron transport chain to DCPIP. Some weed killers are substances that accept electrons during photosynthesis.