Asked by: Mendia Horvath
science genetics

Why is PCR used in the process of DNA sequencing?

Last Updated: 9th April, 2020

26
PCR stands for Polymerase Chain Reaction, and in short, it copies DNA millions of times very quickly. It is used in DNA sequencing because sometimes the DNA sample is too small. This happens, for instance, in crime scene evidence, or in very old samples (eg. mummies).

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Then, why is PCR used in the process of DNA sequencing quizlet?

The PCR is able to replicate the given DNA into millions of copies and use only the smaller pieces of DNA required to make a sequence. This is the ability to isolate strands from virus and compare it to database if desired. You just studied 26 terms!

Similarly, what is the difference between DNA sequencing and PCR? 1 Answer. PCR is a technique used to duplicate DNA artificially. This is done to have enough quantity of it for the next process which is sequencing. DNA sequencing is a process where the sequence of the bases in DNA is determined for medical, criminal or research uses.

Also, what is PCR in DNA sequencing?

Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest.

What is PCR test is used for?

Polymerase chain reaction (PCR) tests are used to detect HIV's genetic material, called RNA. These tests can be used to screen the donated blood supply and to detect very early infections before antibodies have been developed. This test may be performed just days or weeks after exposure to HIV.

Related Question Answers

Waleed Prazeres

Professional

What is PCR What does it do quizlet?

polymerase chain reaction. most widely used amplification technique. enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. in nature, msot organisms copy their DNA in the same way. The PCR mimics this process, only it does it in a test tube.

Jesse Useli

Professional

What is the overall purpose of the polymerase chain reaction PCR?

polymerase chain reaction / PCR. Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

Bihotza Molinares

Professional

What is the function of the high temperature step in PCR polymerase chain reaction )? Quizlet?

-Given that PCR involves very high temperatures, it is imperative that a heat-stable DNA polymerase be used in the reaction. -The PCR reaction will amplify the DNA section between the two primers. This is the basis of genetic counseling and PCR is used as part of the diagnostic tests for genetic diseases.

Houssien Schmiedbauer

Explainer

Why is PCR used in the process of DNA sequencing How can DNA sequencing be used to identify other classes of pathogens such as viruses?

Scientists can identify specific bacteria when they are amplifying and studying the same region of DNA in each species by isolating the DNA, making copies of it, and sequencing it to identify the bacteria. PCR is used in the process of DNA sequencing because it copies the DNA to give different varieties of theDNA.

Kimiko Wenke

Explainer

How can DNA sequencing be used to identify viruses?

Putting DNA to Work - Infectious Disease - Identify the Disease Activity. Every virus has its own DNA sequences. The goal is to probe them all. The identification of the cause of SARS provides an example of the great speed that modern DNA sequencing has brought to the detection of infectious diseases.

Policarpio Serem

Explainer

What are the basic steps for isolating and identifying bacterial DNA?

Basic Steps
Prepare a sample from a patient and isolate whole bacterial DNA. Make many copies of the desired piece of DNA. Sequence the DNA. Analyze the sequence and identify the bacteria.

Faride Rabanedo

Pundit

What is a chain reaction quizlet?

Nuclear Chain Reactions. -it is when absorption of a neutron causes nucleus to break apart. ~lots of energy released. ~released neutrons can then be absorbed by other nuclei.

Nourreddine Avaliani

Pundit

What is the purpose and benefit of the polymerase chain reaction?

What is the purpose and benefit of the polymerase chain reaction? Polymerase Chain Reaction (PCR) is the technique by which one can multiply specific regions of DNA (Deoxyribonucleic Acid). To carry out PCR, a special type of thermostable DNA polymerase is used, Taq polymerase for the replication of strands of DNA.

Anguel Rosquete

Pundit

What are the 3 steps of PCR?

PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.

Deva Strauf

Pundit

What is the principle of PCR?

Working principle of PCR
As the name implies, it is a chain reaction, a small fragment of the DNA section of interest needs to be identified which serves as the template for producing the primers that initiate the reaction. One DNA molecule is used to produce two copies, then four, then eight and so forth.

Xiujuan Vindman

Pundit

What does Taq polymerase do in PCR?

“The function of Taq DNA polymerase in PCR reaction is to amplify the DNA for the production of multiple copies of it. Taq DNA polymerase is a thermostable DNA polymerase which can even work at a higher temperature.”

Berna Bavidoff

Teacher

What are the 4 steps of PCR?

Steps Involved in Polymerase Chain Reaction in DNA Sequence
  • Step 1: Denaturation by Heat: Heat is normally more than 90 degrees Celsius at separates double-stranded DNA into two single strands.
  • Step 2: Annealing Primer to Target Sequence:
  • Step 3: Extension:
  • Step 4: End of the First PGR Cycle:

Custodio Burge

Teacher

What are the four types of dNTPs?

The Role of dNTP
There are four types of dNTP, or deoxynucleotide triphosphate, with each using a different DNA base: adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP).

Oretha Borega

Teacher

What are the different types of PCR?

Some of the common types of PCR are;
  • Real-Time PCR (quantitative PCR or qPCR)
  • Reverse-Transcriptase (RT-PCR)
  • Multiplex PCR.
  • Nested PCR.
  • High Fidelity PCR.
  • Fast PCR.
  • Hot Start PCR.
  • GC-Rich PCR.

Nigel Makhlouf

Teacher

How is PCR used to identify bacteria?

The principle of the method is simple; when a pure PCR product of the 16S gene is obtained, sequenced, and aligned against bacterial DNA data base, then the bacterium can be identified. A selected PCR band from each of 40 isolates was sequenced and the bacterium identified to species or genus level using BLAST.

Dana Charlene

Reviewer

What is the full form of PCR?

polymerase chain reaction

Pritivi Wischuf

Reviewer

What are the four main components of a PCR DNA amplification reaction?

What are the four main components of a PCR DNA amplification reaction? DNA Template, Taq DNA Polymerase, Oligonucleotide Primers, and Nucleotides.

Margo Maurer

Reviewer

What are the steps of DNA sequencing?

What are the steps in DNA sequencing?
  • Sample preparation (DNA extraction)
  • PCR amplification of target sequence.
  • Amplicons purification.
  • Sequencing pre-prep.
  • DNA Sequencing.
  • Data analysis.

Itxaropena Atxurra

Reviewer

Do you need DNA ligase for PCR?

The equivalent of DNA polymerase I and DNA ligase are also unnecessary due to the absence of RNA primers and Okazaki fragments during the process of PCR. Since PCR requires very high temperatures as you will see, a typical DNA polymerase cannot be used since it will be denatured by the intense heat.

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9th April, 2020

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