Asked by: Maple Hormaecheamedical health infectious diseases
Why is the streak plate so important in the production of pure cultures?
Last Updated: 30th March, 2020
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Simply so, what is the importance of the streak plate method?
Streak plate technique is used to grow bacteria on a growth media surface so that individual bacterial colonies are isolated and sampled. Samples can then be taken from the resulting isolated colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
Secondly, why is it important to obtain isolation of bacteria into pure culture? The isolation of bacteria in pure culture is important because it facilitates the application of recombinant DNA technology through the isolation of clones.
Keeping this in view, why is it important to make pure cultures?
The importance of having a pure culture, and not a mixed culture, when performing biochemical testing is that a pure culture may react much differently in isolation than when it is combined with other species. Bacteria replicates at infinitesimally long rates and one species may enforce or weaken the other.
What are three methods commonly used to derive a pure culture?
Such isolated colonies are picked up separately using sterile inoculating loop/needle and re-streaked onto fresh media to ensure purity.
- Pour Plate Method:
- Spread Plate Method:
- Serial Dilution Method:
- Single Cell Isolation Methods:
- Enrichment Culture Method: