Asked by: Romilda Vajipeyajula
medical health infectious diseases

What is SPS additive?

Last Updated: 29th June, 2020

Additive: anticoagulant SPS (Sodium Polyanetholsulfonate) & ACD (acid citrate dextrose) What additive does: Prevents the blood from clotting and stabilizes bacterial growth.

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Hereof, what is an SPS tube?

Where are SPS (sodium polyanethol sulfonate) tubes used? A. SPS tubes are used for blood culture specimen collection in microbiology. Eight gentle tube inversions will prevent the blood from clotting. The blood can remain in the SPS tube for two to four hours before it has to be transferred to a blood culture bottle.

Likewise, what is SPS in phlebotomy? Sodium polyanethole sulfonate (SPS) is the most common anticoagulant used in commercial blood culture bottles. SPS has been shown to function as an anticoagulant (4) and as an inhibitor of humoral and cellular elements that might interfere with bacterial growth.

Moreover, why is SPS used in blood cultures?

Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture media. The addition of SPS to experimental blood cultures inoculated with small numbers of a variety of organisms caused a striking increase in recovery of these organisms.

What are the additives in the tubes?

Tube cap color Additive
Red or gold (mottled or "tiger" top used with some tubes is not shown) Serum tube with or without clot activator or gel
Green Sodium or lithium heparin with or without gel
Lavender or pink Potassium EDTA
Gray Sodium fluoride, and sodium or potassium oxalate

Related Question Answers

MariƱe Vianel


What additive is in a GREY top tube?

Grey-top tube (potassium oxalate/sodium fluoride)
This tube contains potassium oxalate as an anticoagulant and sodium fluoride as a preservative – used to preserve glucose in whole blood and for some special chemistry tests.

Addie Ponciano


What is BD Vacutainer used for?

BD Vacutainer® SST™ Tubes provide an efficient means of serum sample preparation and help to improve laboratory workflow. BD Vacutainer® Plus Plastic Serum Tubes have spray-coated silica and are used for serum determinations in chemistry, serology, and immunohematology.

Mechthild Murguizu


What tests go in what color tubes?

Green top tube with sodium or lithium heparin: used for plasma or whole blood determinations. EDTA tubes: includes Lavender top, Pink top (used for blood bank testing), Tan top (used for lead testing), and Royal Blue top with EDTA (used for trace metal whole blood or plasma determinations).

Mazhar Velt


What tube is used for PT and PTT?

Blue-Top Tube - Sodium Citrate (NaCitrate): This tube is primarily used for coagulation studies (PT and PTT).

Charmaine Rueters


What color tube is used for TSH?

Specimen type: Plasma or serum
Preferred collection container: Stat/Line draws: 3 mL green/yellow-top (plasma separator) tube Routine requests/off-site specimens: 3.5 mL gold-top (serum separator) tube

Yichen Velio


What is the order of draw?

Order of draw is the tube sequence a certified phlebotomist needs to follow while collecting blood. Each tube is differentiated by the tube additive and color. A trained phlebotomist using the correct order of draw ensures they obtain a quality sample to be used for diagnostic purposes to provide accurate results.

Georgianna Poggenhans


What color is BMP tube?

Green Tube/Plasma: Centrifuge after collection. Gold Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Mirjam Morsdorf


What can a blood culture detect?

A blood culture is done to: Find a bacterial infection that has spread into the blood, such as meningitis, osteomyelitis, pneumonia, a kidney infection, or sepsis. A culture can also show what type of bacteria is causing the infection.

Stan Teunesen


Which blood culture bottle do you draw first?

If collecting from an adult or adolescent, the aerobic bottle (blue top) must always be inoculated first to avoid the release of air from the syringe into the anaerobic bottle.

Willene Kuhleber


When Should blood cultures be taken?

Blood cultures should always be requested when a bloodstream infection or sepsis is suspected. Blood cultures should be collected: as soon as possible after the onset of clinical symptoms; ideally, prior to the administration of antimicrobial therapy(16).

Salama Piepjahn


What is in a blood culture bottle?

Method. A minimum of 10 ml of blood is taken through venipuncture and injected into two or more "blood bottles" with specific media for aerobic and anaerobic organisms. A common medium used for anaerobes is thioglycollate broth. The blood is collected using aseptic technique.

Ganna Mittasch


What is the solution in blood culture bottles?

Columbia Broth is particularly recommended for blood culture because of its ability to grow a wide range of microorganisms. Columbia Broth with Increased Cystein, Hemin and Menadione provide improved recovery of anaerobic microorganisms from blood specimens.

Matvey Mogort


What is the purpose of sodium Polyanethole sulfonate?

Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors.

Haiping Poschen


What color tube contains sodium Polyanethol sulfonate?

3 Yellow with clear label 8 to 10 times Sodium polyanethol sulfonate (SPS) Tube used for Mycobacteria (AFB) blood culture.

Yrama Johansson


What is ACD in phlebotomy?

acid-citrate-dextrose (ACD) An anticoagulant containing citric acid, sodium citrate and dextrose. This was formerly used primarily as a whole blood preservative, but is currently used for plateletpheresis.

Leopoldina Schickentanz


What is sodium Polyanethol sulfonate?

Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors.

Shandi Castellana


How do you remember Order of phlebotomy?

“What mnemonic do you use to remember or teach the proper order of draw? (example: "Studious Boys Rarely Get Low Grades" = Sterile, blue, red, green, lavender, gray.)”

Leonardo Povoa


What do the colors of blood test tubes mean?

The tests each bottle is used for are the same: the purple one is for cell count, the yellow one is for electrolytes, albumin and LDH, the grey one is for glucose, and blood culture bottles can be used for fluid cultures.

Augustus Peterhans